• 
• Eileen Atkins
• Elizabeth I
  

• 
• Claire Bloom
• Mary Queen of Scots
  

• 
• Norman Allen
• Cleric
  Francois II
  Ormiston
  

• 
• Gaetano Bongiovanni
• David Rizzio
  Pope
  

• 
• Ralph Clanton
• Claud Nau
  

• 
• Peter Coffield
• Lord Darnley
  

• 
• Noel Craig
• Davison
  

• 
• John Devlin
• Cardinal of Lorraine
  Sir Francis Walsingham
  

• 
• Ralph Drischell
• Prisoner
  Tala
  

• 
• Robert Elston
• Robert Dudley
  

• 
• Dillon Evans
• de Quadra
  

• 
• Joseph Hill
• Lindsey
  

• 
• Diana Kirkwood
• Catherine de Medici
  First Court Lady
  

• 
• Randy Levey
• Bagpiper
  Jailer
  One of the Courtiers, Lairds, Clerks, Servants, etc.
  

• 
• Stephen Macht
• Brewer
  Jailer
  One of the Courtiers, Lairds, Clerks, Servants, etc.
  

• 
• Don McHenry
• Bishop of Durham
  Lord Mor
  

• 
• Douglas Rain
• William Cecil
  

• 
• Lee Richardson
• Lord Bothwell
  

• 
• Stephen Scott
• Lord Morton
  

• 
• Alexander Scourby
• John Knox
  

• 
• Jane Singer
• Second Court Lady
  

• 
• Brian Sturdivant
• One of the Courtiers, Lairds, Clerks, Servants, etc.
  Philip II
  

• 
• Ian Sullivan
• One of the Courtiers, Lairds, Clerks, Servants, etc.
  Ruthven
  

• 
• Theodore Tenley
• Doctor
  One of the Courtiers, Lairds, Clerks, Servants, etc.
  Scots Archibishop
  

• 
• John Cullum
• Lord Bothwell
  

• 
• Norman Allen
• de Quadra
  

• 
• Ralph Drischell
• John Knox
  William Cecil
  

• 
• John Handy
• Cleric
  David Rizzio
  Francois II
  Ormiston
  Pope
  

• 
• Joseph Hill
• Lord Morton
  

• 
• Diana Kirkwood
• Elizabeth I
  

• 
• Randy Levey
• Cleric
  Francois II
  Ormiston
  

• 
• Stephen Macht
• Cardinal of Lorraine
  Sir Francis Walsingham
  

• 
• Don McHenry
• Claud Nau
  

• 
• Jane Singer
• Mary Queen of Scots
  

• 
• Brian Sturdivant
• Lord Darnley
  Robert Dudley
  

• 
• Ian Sullivan
• Lord Bothwell
  

• 
• Robert Bolt
• Writer
  

• 
• Peter Dews
• Director
  

• 
• Carl Toms
• Scenic Design
  

• 
• Lloyd Burlingame
• Lighting Design
  

• 
• Mitchell Erickson
• Production Stage Manager
  

• 
• John Handy
• Stage Manager
  

• 
• Arthur Cantor
• Press Representative
  Producer
  

• 
• Ellen Levene
• Press Representative
  

• 
• David Merrick
• Producer
  

• 
• H.M. Tennent, Ltd.
• Produced by arrangement with
  

• 
• Samuel Liff
• Associate Producer